Table 3.

ANOVA for the response surface quadratic model.

Source	Sum of		Mean	F	p-value
	Squares	df	Square	Value	Prob > F
Model	576.98	14	41.21	835.76	< 0.0001	significant
A-pH	28.12	1	28.12	570.23	< 0.0001
B-Time	3.33	1	3.33	67.62	0.0002
C-Nano	0.25	1	0.25	5.12	0.0644
D-Concentration	72.00	1	72.00	1460.10	< 0.0001
AB	0.069	1	0.069	1.40	0.2817
AC	0.13	1	0.13	2.54	0.1622
AD	0.11	1	0.11	2.20	0.1887
BC	0.032	1	0.032	0.64	0.4527
BD	0.32	1	0.32	6.54	0.0431
CD	0.31	1	0.31	6.37	0.0450
A2	0.48	1	0.48	9.79	0.0203
B2	6.59	1	6.59	133.64	< 0.0001
C2	0.14	1	0.14	2.78	0.1465
D2	10.39	1	10.39	210.79	< 0.0001
Residual	0.30	6	0.049
Lack of Fit	0.19	2	0.097	3.78	0.1198	not significant
Pure Error	0.10	4	0.026
Cor Total	577.28	20

R² = 0.9995, Adj. R² = 0.9983, Pred. R² = 0.9233, Adequate precision = 113.257.