Skip to main content
. 2019 Aug 14;13:53. doi: 10.3389/fncir.2019.00053

Figure 2.

Figure 2

Anxiety behavior of transgenic zebrafish overexpressing spx1 in the dHb and wildtype siblings. The novel tank test was used to assess the anxiety-related behaviors in adult Tg(gpr151:gal4vp16);Tg(5xuas:spx1:p2a-mCherry) zebrafish (dHb-spx1 OV; n = 38) and their wildtype siblings (n = 38). (A) Representative behavior-tracking results for 2 min at 2 and 10 min after diving. The black quadrangular boxes and blue dotted lines indicate the novel tanks and half lines of tanks. The red lines indicate the tracing lines of the moving fish. (B–D) Graph bars and error bars are expressed as mean ± standard error of the mean (SEM). White or black dots in the graphs indicate individual data points. (B) The mean velocity at 2 min at 2 and 10 min after diving [2 min: siblings, 4.365 ± 0.3378 cm/s, dHb-spx1 OV, 4.565 ± 0.3316 cm/s; 10 min (median): siblings, 4.816 cm/s; dHb-spx1 OV, 5.323 cm/s. n.s., not statistically significant]. The unpaired t-test was used for the 2-min analysis and the Mann-Whitney U-test for the 10-min analysis. (C) Time spent at the top half of the tank for 2 min, at 2 min and 10 min after diving [2 min (median): siblings, 0.02179 s, dHb-spx1 OV, 11.56 s; 10 min (median): siblings, 19.5 s, dHb-spx1 OV, 48.88 s; *p < 0.05, **p < 0.005, Mann-Whitney U-test]. (D) The number of fish that entered the top half of the tank for 2 min, at 2 min and 10 min after diving [2 min (median): siblings, 0.5; dHb-spx1 OV, 3.5. 10 min (median): siblings, 6.5; dHb-spx1 OV, 15.5; *p < 0.05, n.s., not significant (Mann-Whitney U-test)]. (E) The latency to enter the top half of the tank. (Median: siblings, 134.4 s; dHb-spx1 OV, 65.17 s; *p < 0.05, Mann-Whitney U-test).