Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 14;10:1909. doi: 10.3389/fimmu.2019.01909

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Wang, Tsai, Ko, Yang, Lin, Hung and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

B10 cells lacking Blimp-1 failed to suppress T-cell proliferation. (A) Experimental design for the co-culture of B10 cells with anti-CD3-and anti-CD28-stimulated naïve CD4⁺ T cells. CFSE-labeled CD4⁺ T cells in the presence of anti-CD3 and anti-CD28 antibody were co-cultured with B10 cells from mice of the indicated genotypes for 3 days and then analyzed for CFSE dilution by FACS. (B) FACS analysis showing the frequency of dividing CFSE-labeled CD4⁺ T cells. Data are representative from three independent biological replicates. (C) Statistical results from three experiments in (B) are shown. Data were analyzed using an unpaired Student's t-test and are the mean ± SEM (n = 3 mice per group). ^*p < 0.05 and ^***p < 0.001. (D) GO analysis showed a significant enrichment for genes in “T cell selection” and “leukocyte migration” in the category of immune system process within the Biological Process ontology in anti-CD40 stimulated Cko B10 cells as compared with genes in anti-CD40 stimulated Ctrl B10 cells. The significant terms in the immune system process within the Biological Process ontology were all listed.