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. 2019 Aug 14;12:198. doi: 10.3389/fnmol.2019.00198

FIGURE 1.

FIGURE 1

Primary cell cultures display normal expression of cellular and synaptic markers at DIV24-27. (A) Representative immunocytochemistry images of DIV 21-27 neurons show the colocalization of the presynaptic protein synapsin-1 (red) and the postsynaptic AMPA receptor subunit GluA1 (green) with MAP2-positive neurons (magenta) and nuclei (DAPI; blue) (scale bar = 50 μm). Lower panels show further magnified dendritic compartments (100 μm) from Synapsin-1 (top), GluA1 (middle) and the colocalization of both (bottom; scale bar = 10 μm). Primary cell cultures also show populations of (B) GFAP-positive astrocytes (magenta) closely associating with GluA1-positive neurons (green). Inset images show further magnified somatic compartments. (C) Representative electron micrograph showing the presence of synapses between neighboring primary hippocampal cells in culture. Pre- and postsynaptic regions were observed separated by a synaptic cleft. Pre, presynaptic terminal; Post, postsynaptic region; PRs, polyribosomes; PSD, postsynaptic density; SC, synaptic cleft; M, mitochondria. Scale bar = 100 nm.