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. 2019 Aug 14;12:198. doi: 10.3389/fnmol.2019.00198

FIGURE 3.

FIGURE 3

sAPPα increases somatic and dendritic expression of de novo Arc protein. Representative images show neurons (MAP2 positive neurons; magenta) expressing FUNCAT-PLA signal (green puncta) representing newly synthesized Arc protein in (A) no-drug control cells, (B) anisomycin-treated, and (C) sAPPα-treated (1 nM, 2 h) primary hippocampal cultures. Nuclei are stained blue (DAPI). The relative integrated intensity of (D) somatic and (E) dendritic signal from treatment groups is expressed as drug/average of control and presented as mean ± SEM (n = 25–34 cells from three independent experiments). Co-incubation of AHA with anisomycin inhibited Arc protein synthesis. Incubation with sAPPα significantly increased the expression of de novo synthesized Arc protein in the somata and dendrites. Images show whole cell (scale bar = 50 μm), and magnified somatic (inset, bottom right) and dendritic (50 μm; lower panels; scale bar = 10 μm). Outliers were removed from each experiment prior to amalgamation using Grubb’s tests, and normality was detected by D’Agostino and Pearson omnibus normality tests. Significance was calculated on data expressed relative to control by use of one sample t-tests; hashes (#) indicate significance between control and sAPPα-treated, asterisks (∗) indicate significance between control and anisomycin treated, #p = 0.038, ∗∗∗/### p ≤ 0.0001.