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. 2019 Aug 14;12:198. doi: 10.3389/fnmol.2019.00198

FIGURE 7.

FIGURE 7

Arc protein expression in response to sAPPα is affected by kinase inhibitors. Representative images showing (A) sAPPα (1 nM, 2 h, n = 125) promotes an increase in the expression of Arc protein in cultured neurons relative to (B) no drug controls (n = 134). Cells were simultaneously treated with (C) KN62 (n = 51), (D) PD98059 (n = 51), and (E) KT5823 (n = 42), (F) H-89 dihydrochloride (n = 40), (G) Chelerythrine chloride (n = 30), or (H) Rapamycin (n = 40) and Arc levels in the (I) somata and (J) dendrites was measured. Data are expressed as mean ± SEM from ≥3 experiments. Significance was calculated using a Kruskal–Wallis one-way ANOVA with Dunn’s multiple comparisons test. Hashes (#) indicate significance between control and sAPPα-treated; asterisks (∗) indicate significance between sAPPα- and inhibitor-treated; ####p ≤ 0.0001, ∗p = 0.0428, ∗∗∗p = 0.0001, ∗∗∗∗p ≤ 0.0001. Representative images show neurons (magenta), Arc protein (green), DAPI (blue) (scale bar = 50 m), and magnified somatic (inset, bottom right) and dendritic (100 μm; lower panels; scale bar = 10 μm) compartments.