Skip to main content
. 2019 Aug 14;13:861. doi: 10.3389/fnins.2019.00861

FIGURE 4.

FIGURE 4

LTP induced by three strong sets of 400 Hz HFS is both NMDA receptor- and VGCC-dependent in freely behaving TG rats. (A) Pretreatment with either the NMDA receptor antagonist CPP (2 h prior to 3 × s400 Hz HFS, i.p.) or the VGCC inhibitor mibefradil (MBF, 30 min prior to 3 × s400 Hz HFS, i.c.v.) alone only partly inhibited LTP induction. (B) Pretreatment with the combination of these agents completely inhibits LTP in TG animals. Left-hand panels show LTP time course. Inserts show representative EPSP traces at the times indicated. Calibration bars: vertical, 1 mV; horizontal, 10 ms. The time point of HFS application is indicated by three arrow heads. Summary bar charts of LTP (last 10 min post-HFS) are in right hand panel. The # symbol stands for a statistical comparison between pre- and 3 h post-HFS values within one group (paired t-test) whereas an * indicates a comparison of 3 h post-HFS values between groups [one-way ANOVA followed by post hoc Bonferroni tests in (A) or unpaired t-test in (B)]. One symbol, p < 0.05; two symbols, p < 0.01; three symbols, p < 0.001; ns, p > 0.05. Values are mean ± S.E.M.% pre-HFS baseline EPSP amplitude.