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. 2019 Aug 14;10:556. doi: 10.3389/fendo.2019.00556

Table 1.

Primers used in the present study.

Gene Forward primer (5'-3') Reverse primer (3'-5') AT
PRIMERS FOR FULL-LENGTH PCR
tac3a ATGAGGAGAGGATTGCTGTTG CTACAGCCCCTGAAGAAACC 56
tac3b ATGGAGAGAACTCCAAACTG CTACAGAAAGCGTTGAAACC 56
tacr3a ATGGCGGCTCCACACAAC TCAAGAGAACTCCTCAGGTT 56
tacr3b ATGGCCTCTGAACGGGAT CTAACTGCCTTTATTATTGG 55
PRIMERS FOR REAL-TIME PCR
tac3a GAGGAGAGGATTGCTGTTGGTGAC TCCAAGCCTACGGTCGGATCTG 61
tac3b TTGCTGCGGAACACAAGGATGG AACCTCTGCCTGCCATGATTGATG 60
tacr3a AACCTCTGCCTGCCATGATTGATG CCGTGTGGCGAGATGTGATGTAG 62
tacr3b CAAGCGGATGCGGACTGTCAC CTAACTGCCTTTATTATTGG 61
Motilin (mln) TGCTGATGAAGGAGCGAGAA TCCACCATGTTCCACCTGAG 56
Ghrelin (ghrl) ACACCTGTTTGCTGGTCTTTC ATGTGATGTGGTTGGCCTCTG 56
Gastrin (gas) TGCTAAGAGGGAGAAACTG TATCTCGCGTTCATCGTC 55
Cholecystokinin (cck) TGCCAACTACAACCAACCT GCGTCGTCCAAAGTCCAT 56
ghrh GGCCGGAGGGATACTTCCAT GCAGAGATTTGGCCCAGGAC 59
prlh TGCTCCTCCTCCTCCTCTCCTC CACGCTGCTGTGCCTCTTCC 62
gh AAGAGTGGTCCTCCTGCTGTCC TTGTTGAGTTGACGCTGCTCCTC 61
igf1 CATTGTGGACGAGTGCTGCTTCC CTTGTCTGGCTGCTGTGCTGT 61
18S GGGTCCGAAGCGTTTACT TCACCTCTAGCGGCACAA 56