Table 1.
Gene | Forward primer (5'-3') | Reverse primer (3'-5') | AT |
---|---|---|---|
PRIMERS FOR FULL-LENGTH PCR | |||
tac3a | ATGAGGAGAGGATTGCTGTTG | CTACAGCCCCTGAAGAAACC | 56 |
tac3b | ATGGAGAGAACTCCAAACTG | CTACAGAAAGCGTTGAAACC | 56 |
tacr3a | ATGGCGGCTCCACACAAC | TCAAGAGAACTCCTCAGGTT | 56 |
tacr3b | ATGGCCTCTGAACGGGAT | CTAACTGCCTTTATTATTGG | 55 |
PRIMERS FOR REAL-TIME PCR | |||
tac3a | GAGGAGAGGATTGCTGTTGGTGAC | TCCAAGCCTACGGTCGGATCTG | 61 |
tac3b | TTGCTGCGGAACACAAGGATGG | AACCTCTGCCTGCCATGATTGATG | 60 |
tacr3a | AACCTCTGCCTGCCATGATTGATG | CCGTGTGGCGAGATGTGATGTAG | 62 |
tacr3b | CAAGCGGATGCGGACTGTCAC | CTAACTGCCTTTATTATTGG | 61 |
Motilin (mln) | TGCTGATGAAGGAGCGAGAA | TCCACCATGTTCCACCTGAG | 56 |
Ghrelin (ghrl) | ACACCTGTTTGCTGGTCTTTC | ATGTGATGTGGTTGGCCTCTG | 56 |
Gastrin (gas) | TGCTAAGAGGGAGAAACTG | TATCTCGCGTTCATCGTC | 55 |
Cholecystokinin (cck) | TGCCAACTACAACCAACCT | GCGTCGTCCAAAGTCCAT | 56 |
ghrh | GGCCGGAGGGATACTTCCAT | GCAGAGATTTGGCCCAGGAC | 59 |
prlh | TGCTCCTCCTCCTCCTCTCCTC | CACGCTGCTGTGCCTCTTCC | 62 |
gh | AAGAGTGGTCCTCCTGCTGTCC | TTGTTGAGTTGACGCTGCTCCTC | 61 |
igf1 | CATTGTGGACGAGTGCTGCTTCC | CTTGTCTGGCTGCTGTGCTGT | 61 |
18S | GGGTCCGAAGCGTTTACT | TCACCTCTAGCGGCACAA | 56 |