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. 2019 Aug 14;10:1925. doi: 10.3389/fimmu.2019.01925

Figure 5.

Figure 5

ZIKV infection induced NLRP3 inflammasome activation in the kidneys. (A,B) The relative mRNA levels (vs. mock group) of NLRP1, NLRP3, IPAF, and AIM2 in the kidneys of mock and ZIKV-infected newborn (A) and adult (B) mice at 7 days post-infection were detected by qRT-PCR. (C–I) Expression levels of NLRP3 (D), ASC (E), pro-caspase-1 (F), cleaved-caspase-1 (G), pro-IL-1β (H), and mature IL-1β (I) in the kidneys of newborn and adult mice at 7 days post-infection were assessed by western blot. (J,K) Examination of mature IL-1β release in the supernatants of mouse kidneys at 7 days post-infection (n = 6 per group) were determined by an enzyme-linked immunoadsorbent assay (ELISA). Data were presented as mean ± SEM (n = 6), *p < 0.05 vs. mock group; #p < 0.05 vs. ZIKV group (***p < 0.001 vs. mock group or ZIKV+MCC950 group in Figure J and K).