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. 2019 Aug 14;10:1925. doi: 10.3389/fimmu.2019.01925

Figure 8.

Figure 8

Overexpression of Bcl-2 down-regulated the expression of cleaved caspase-3 and PARP in ZIKV-infected renal cells. (A,B) Cell apoptosis was tested in the kidneys of ZIKV- infected newborn (A) and adult mice (B) at 14 days post-infection using a TUNEL assay kit. Positive apoptotic cells detected by the DAB reagent had a brownish-yellow nucleus, which indicated by black arrows. Scale bars, 20 μm. (C,D) The expression of Bcl-2, cleaved caspase-3, and cleaved PARP in the kidneys of newborn mice were assessed by western blot. (E,F) HK-2 cells were transfected with control vector or pSG5-FLAG-Bcl-2 plasmid (2 μg/ml) for 24 h followed by incubation with PBS (mock group) or ZIKV (MOI = 2) for 24 h. Expression levels of FLAG, cleaved caspase-3 and cleaved PARP protein at 0 h and 24 h post-infection were assessed by western blot. Data were presented as mean ± SEM, (***p < 0.001 vs. mock or vector group).