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. 2019 Aug 14;13:346. doi: 10.3389/fncel.2019.00346

FIGURE 2.

FIGURE 2

Mutant SOD1 but not wild type SOD1 interacts with VDAC1 and inhibits channel conductance. (A,B) Recombinant full length VDAC1 purified from E. coli was reconstituted into a PLB and channel currents through VDAC1, in response to a voltage step from 0 to 10 mV (A) or to –10 mV (B), before and 15–20 min after addition of 40 μg/ml (final concentration) of SOD1 were recorded. (C) VDAC1 relative conductance as a function of voltage in a 60 to –60 mV step before (∙) and after addition of SOD1WT (∘). Relative conductance (conductance/maximal conductance) was determined as the average steady-state conductance at a given voltage normalized to the conductance at 10 mV, considered the maximal conductance. (D) Histogram of VDAC1 current amplitudes at 10 mV before and after addition of SOD1WT. (E–H) Similar experiments as in (A–D), except that mutant SOD1G93A was used. (I–L) Similar experiments as in (A–D), except that mutant SOD1G85R was used. (M) Coomassie blue staining of recombinant full length and residue 1-26-truncated VDAC1 (ΔN-VDAC1).