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. 2019 Aug 14;13:346. doi: 10.3389/fncel.2019.00346

FIGURE 6.

FIGURE 6

The VDAC1 N-terminal peptide ameliorates SOD1G93A-mediated motor neuron toxicity. SOD1G93A-expressing mESC-derived MNs were incubated with the indicated concentrations of (1-20)N-Ter-Antp peptide for 1–4 days following initiation of final differentiation. (A,B) (1-20)N-Ter-Antp at a concentration of 10 μM significantly increased MN neurite outgrowth over the first 24 h. Scale bar, 100 μm. (C) Twenty-four hours after the initiation of differentiation, 5 and 10 μM of (1-20)N-Ter-Antp peptide significantly increased the number of MNs present in the culture. (D) (1-20)N-Ter-Antp at a concentration of 10 μM significantly increased the number of surviving MNs over the course of final differentiation. After 96 h, MNs showed the typical cellular morphology of maturing neurons with extension of a singular long process and branching resembling a dendritic tree. GFP fluorescence indicates the expression of the motor neuron-specific transcription factor HB9. Gross cellular morphology of surviving MNs remained unaffected by the (1-20)N-Ter-Antp peptide. Scale bar, 25 μm. (E) Quantification of MNs survival. For all assays, results are expressed as the mean ± SEM (n = 3). *P < 0.05.