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. 2019 Aug 14;10:1930. doi: 10.3389/fimmu.2019.01930

Figure 4.

Figure 4

ERK1/2 and p38 are essential to MKP1-mediated macrophage polarization. (A) ERK1/2 and p38 are involved in the expression of iNOS and Arg-1. Cells were pretreated with 20 μM PUN with desired chemical inhibitors for 3 h and then treated with 1 μg/mL of LPS for 12 h, as indicated. The expression of iNOS and Arg-1 were detected by Western blot. Data are expressed as the mean ± SD of three independent experiments. **p < 0.01 and ##p < 0.01 indicate statistically significant differences compared with the LPS group. $p < 0.05 indicates statistically significant differences compared with LPS/PUN group. (B) ERK1/2 and p38 are involved in the expression of IL-1β and IL-10. Cells were pretreated with 20 μM PUN with the desired chemical inhibitors for 3 h and then treated with 1 μg/mL of LPS for 24 h, as indicated. The expression of IL-1β and IL-10 were detected by ELISA. Data are expressed as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 indicate statistically significant differences compared with LPS group. $p < 0.05 indicates statistically significant differences compared with LPS/PUN group.