Figure-5.
Amplification plot of real-time polymerase chain reaction showed positive results for curve numbers 2, 3, 5, and 6 for DNA extracted from field samples skin biopsy and its related blood of infected cattle before virus isolation. Curve 7: DNA from positive chorioallantoic membrane with pock lesion of the isolated virus. Curve 4: Ct of DNA template positive control of lumpy skin disease virus. Curve 1: DNA-free sample negative control (graph generated by ES Equant tube scanner software).