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. 2019 Jul 8;12(7):984–993. doi: 10.14202/vetworld.2019.984-993

Figure-4.

Figure-4

Polymerase chain reaction (PCR) amplification of antibiotic resistance genes of Escherichia coli and Salmonella spp. (a) PCR amplification of ereA gene of erythromycin resistant E. coli and Salmonella spp. Lane M: 100 bp DNA Marker, 1: Negative control, 2-3: Representative E. coli isolates, 4-5: Representative Salmonella spp. isolates, 6: Positive control for E. coli, and 7: Positive control for Salmonella spp. (b) PCR amplification of tetA gene of tetracycline resistant E. coli and Salmonella spp. Lane M: 100 bp DNA Marker, 1: Negative control, 2-3: Representative E. coli isolates, 4-5: Representative Salmonella spp. isolates, 6: Positive control for E. coli, and 7: Positive control for Salmonella spp. (c) PCR amplification of tetB gene of tetracycline resistant E. coli and Salmonella spp. Lane M: 100 bp DNA Marker, 1: Negative control, 2-3: Representative E. coli isolates, 4-5: Representative Salmonella spp. isolates, 6: Positive control for E. coli. and 7: Positive control for Salmonella spp. (d) PCR amplification of SHV gene of ertapenem, imipenem and meropenem resistant E. coli and Salmonella spp. Lane M: 100 bp DNA Marker, 1: Negative control,2: Positive control for E. coli, 3: Positive control for Salmonella spp., 4-5: Representative E. coli isolates, and 6-7: Representative Salmonella spp. isolates.