Table 1.
Target genes | Primer sequence(5’- 3’) | Approximate band size(bp) | Annealing temperature(°C) | References |
---|---|---|---|---|
E.coli 16S rRNA | F: GACCTCGGTTTAGTTCACAGA R: CACACGCTGACGCTGACCA |
585 | 55 | [21] |
invA | F: ATCAGTACCAGTCGTCTTATCTTGAT R: TCTGTTTACCGGGCATACCAT |
211 | 58 | [22] |
st×1 | F: ACAATCAGGCGTCGCCAGCGCACTTGCT R: TGTTGCAGGGATCAGTGGTACGGGGATGC |
606 | 58 | [23] |
st×2 | F: CCACATCGGTGTCTGTTATTAACCACACC R: GCAGAACTGCTCTGGATGCATCTCTGGTC |
372 | 58 | [23] |
rfbO157 | F: AAGATTGCGCTGAAGCCTTTG R: CATTGGCATCGTGTGGACAG |
497 | 66 | [24] |
ereA | F: GCCGGTGCTCATGAACTTGAG R: CGACTCTATTCGATCAGAGGC |
419 | 52 | [27] |
tetA | F: GGTTCACTCGAACGACGTCA R: CTGTCCGACAAGTTGCATGA |
577 | 57 | [28] |
tetB | F: CCTCAGCTTCTCAACGCGTG R: GCACCTTGCTGATGACTCTT |
634 | 56 | [28] |
SHV | F: TCGCCTGTGTATTATCTCCC R: CGCAGATAAATCACCACAATG |
768 | 52 | [27] |
E. coli=Escherichia coli