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. 2019 Jul 8;12(7):984–993. doi: 10.14202/vetworld.2019.984-993

Table 1.

List of primers used.

Target genes Primer sequence(5’- 3’) Approximate band size(bp) Annealing temperature(°C) References
E.coli 16S rRNA F: GACCTCGGTTTAGTTCACAGA
R: CACACGCTGACGCTGACCA
585 55 [21]
invA F: ATCAGTACCAGTCGTCTTATCTTGAT
R: TCTGTTTACCGGGCATACCAT
211 58 [22]
st×1 F: ACAATCAGGCGTCGCCAGCGCACTTGCT
R: TGTTGCAGGGATCAGTGGTACGGGGATGC
606 58 [23]
st×2 F: CCACATCGGTGTCTGTTATTAACCACACC
R: GCAGAACTGCTCTGGATGCATCTCTGGTC
372 58 [23]
rfbO157 F: AAGATTGCGCTGAAGCCTTTG
R: CATTGGCATCGTGTGGACAG
497 66 [24]
ereA F: GCCGGTGCTCATGAACTTGAG
R: CGACTCTATTCGATCAGAGGC
419 52 [27]
tetA F: GGTTCACTCGAACGACGTCA
R: CTGTCCGACAAGTTGCATGA
577 57 [28]
tetB F: CCTCAGCTTCTCAACGCGTG
R: GCACCTTGCTGATGACTCTT
634 56 [28]
SHV F: TCGCCTGTGTATTATCTCCC
R: CGCAGATAAATCACCACAATG
768 52 [27]

E. coli=Escherichia coli