Figure 4.

a–d) The toxicity of GO to hCorECs. a,b) WST‐8 assay analysis of viability in hCorECs exposed to GO for 2 and 24 h, respectively. c,d) Flow cytometry analysis of apoptosis in hCorECs exposed to GO (50 mg mL⁻¹) for 2 and 24 h, respectively. All the assays were conducted in three independent experiments. Data are presented as mean ± SEM. *p < 0.05. **p < 0.01. e–g) Schematic illustration of the experiments on evaluation of ocular irritation potential of GO exposure using e) in vitro and f,g) in vivo models. Reproduced with permission.103 Copyright 2016, Taylor & Francis.