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. 2019 Jun 14;68(9):1806–1818. doi: 10.2337/db19-0349

Figure 2.

Figure 2

Pdx1, but not Sox9 or Ptf1a, interacts with Brg1 and Brm in wild-type e12.5 pancreatic epithelium. PLA was performed with antibodies specific for Brg1 (A) or Brm (B) and Sox9, Ptf1a, or Pdx1. Distinct fluorescence PLA signals were easily visible in the Pdx1:Brg1 and Pdx1:Brm experiments, but were nearly absent in the Brg1:Sox9, Brg1:Ptf1a, Brm:Sox9, and Brm:Ptf1a assays. The yellow square demarks the magnified area displayed below, and the dashed yellow marks outline the pancreatic epithelium. RBC, red blood cell. Scale bar = 10 μm.