Figure 7.

Tyrosol induces mature blood vessels formation and blood perfusion recovery in diabetic HLI model mice. (A–B) Blood perfusion recovery in the ischemic and non-ischemic hindlimb of diabetic HLI model mice injected by tyrosol: representative of Laser Doppler Perfusion Images (A); and quantification of the blood perfusion ratio at day 0 and day 14 (B) (n = 5, P value was analyzed by one-way ANOVA) were shown. (C–D) Immunofluorescence staining of PECAM-1 (green) and α-SMA (red) in the gastrocnemius muscle of the ischemic hindlimbs of diabetic HLI model mice injected by tyrosol at 14 days post-surgery. Representative images (C; scale bars, 200 µm); and quantification results of PECAM-1 and α-SMA-positive areas (D; n = 6) were shown. Tyrosol and PBS were administered by local injection intramuscularly. Quantification data were expressed as mean ± SD (**P < 0.01); Con, mice injected with PBS; Tyr, mice injected with tyrosol.