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. 2019 Aug 16;9(9):334. doi: 10.1007/s13205-019-1865-7

Fig. 1.

Fig. 1

a Target MYMIV-AC2 region selected for the development of shRNA-based gene-silencing vector. b Schematic map of the RNA-silencing construct pFGC5941::AC2-ihp developed to downregulate the expression of MYMIV-sb-encoded AC2 gene (restriction enzymes NcoI and AscI were used to clone the selected AC2 DNA piece in sense direction, whereas BamHI and XbaI were used to clone antisense direction, respectively; the two AC2 DNA pieces are separated by CHSA intron)