Table 1.

List of primers used in this investigation (sequence details, target region for amplification and the purpose oligonucleotides used are mentioned)

S. no.	Primers	Primer sequences (5′–3′)	Viral genomic region	Purpose
1	AC2-Ihp-F	CATGCCATGGTCTAGAGT GGTATCCCCACCATCTTT	AC2 ORF (transcriptional activator protein—TrAP)	Generation of inverted repeats (RNAi vector)
2	AC2-Ihp-R	CGGGCGCGCCGGATCCGA ACCTTGATTGACGGAGGA
5	qPCR-v-F	ATGTGGGATCCATTGTTG AACG	AV1 ORF (coat protein)	MYMIV titre quantification
6	qPCR-v-R	TCAATCTCCTCCGTGCAT TCG
7	q-AC2-RT-F	GATTTTCGCACAGGGGAG TA	AC2 ORF (transcriptional activator protein—TrAP)	Quantification of MYMIV AC2 gene silencing
8	q-AC2-RT-R	GCTTCAAGTCCAGGAAGCAC
9	F-CHSA	ACTAACTTTGTGGAACTAAA	Complementary to chalcone synthase intron in pFGC5941	PCR verification of inverted repeats (RNAi) vector construction