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Assessment of the chimera formation ability of TSCs by aggregation with eight-cell embryos. A) Images of chimeric blastocysts at 40–48 h after aggregation. Most EGFP-positive TSC-derived cells, if present, were distributed to the polar TE or ICM. Right side: representative images of blastocysts containing cells derived from type 1 colonies. B) The proportions of blastocysts classified by the distribution pattern of TSC-derived cells.
