KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Dylight 649 anti-mouse | Jackson ImmunoResearch Laboratories | 715–495-151 |
| Cy3 anti-rabbit | Jackson ImmunoResearch Laboratories | 111–165-144 |
| Cy3 anti-mouse | Jackson ImmunoResearch Laboratories | 715–165-151 |
| Cy5 anti-rabbit | Jackson ImmunoResearch Laboratories | 711–175-152 |
| mouse monoclonal anti-CENP-A | Enzo Life Sciences | ADI-KAM-CC006-E |
| rabbit anti-CENP-C | [49] | N/A |
| mouse monoclonal anti-MAD2 | Santa Cruz Biotechnology | sc-65492 |
| rabbit anti-CENP-L | [23] | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| CF1.2/1.3 holey carbon grids | EMS | CFT413–50 |
| Uranyl acetate | EMS | 22400 |
| STLC | Sigma | 164739 |
| VectaShield | Vector Laboratories | H-1000 |
| mineral oil | Sigma | M531 |
| RO-3306 | Enzo Life Sciences | ALX-270–463 |
| BS3 | Thermo Fisher Scientific | 21580 |
| Trypsin | Promega corporation | V5111 |
| Glutaraldehyde | Sigma | G5882–10x |
| Iodoacetamide | Sigma | I1149–5G |
| Deposited Data | ||
| CENP-A nucleosome bound by two copies of CENP-CCD and two copies CENP-NNT | This paper | EMDB:9251 |
| CENP-A nucleosome bound by two copies of CENP-CCD and one copy CENP-NNT | This paper | EMDB:9250 |
| CENP-A nucleosome bound by two copies of CENP-CCD and two copies CENP-NNT with local refinement | This paper | EMDB:92523 |
| Model of CENP-A nucleosome bound by two copies of CENP-CCD and two copies CENP-NNT | This paper | PDB:6MUP |
| Model of CENP-A nucleosome bound by two copies of CENP-CCD and two copies CENP-NNT | This paper | PDB:6MUO |
| CCNC BS3 crosslink Mass spectrometry proteomics data | This paper | PXD12605 and 10.6019/PXD12605 |
| Experimental Models: Cell Lines | ||
| DLD-1 Flp-In T-Rex | [48] | N/A |
| DLD-1 CENP-NAID-EGFP/AID-EGFP | [23] | N/A |
| Software and Algorithms | ||
| COOT | [61] | http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot |
| PHENIX | [60] | https://www.phenix-online.org |
| PyMOL | [64] | http://www.pymol.org |
| MotionCor2 | [56] | http://msg.ucsf.edu/em/software/motioncor2.html |
| Gctf | [57] | https://www.mrc-lmb.cam.ac.uk/kzhang/Gctf/ |
| Monores | [59] | https://www.ncbi.nlm.nih.gov/pubmed/29395788 |
| RELION | [58] | http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page |
| UCSF Chimera | [62] | https://www.cgl.ucsf.edu/chimera/ |
| UCSF ChimeraX | [65] | https://www.cgl.ucsf.edu/chimerax/ |
| Curves+ | [69] | https://bisi.ibcp.fr/tools/curves_plus/ |
| LAS-AF | Leica Microsystems | https://www.leica-microsystems.com |
| Fiji (Fiji Is Just ImageJ) | [50] | https://imagej.net/Fiji |
| CRaQ v1.12 | [51] | http://facilities.igc.gulbenkian.pt/microscopy/macros/CRaQ_v1.12.ijm |
| Serial EM | [55] | http://bio3d.colorado.edu/SerialEM/ |
| Find_XL | [66] | http://biolchem.huji.ac.il/nirka/software.html |
| RStudio | [70] | http://www.rstudio.com/. |
| Other | ||
| HisTrap FF | GE Healthcare | 11–0004-58 |
| Amicon Ultra concentrators | Millipore | UFC501024 |
| C18 stage-tips | Thermo Fisher Scientific | 89870 |