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. 2019 Aug 14;10:1905. doi: 10.3389/fimmu.2019.01905

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Copyright © 2019 Yasamut, Thongkum, Moonmuang, Sakkhachornphop, Chaiwarith, Praparattanapan, Wipasa, Chawansuntati, Supparatpinyo, Lai and Tayapiwatana.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Binding and neutralizing activity of mouse anti-IFN-γ mAbs against IFN-γ. (A) The binding activity of the mouse mAbs was determined by indirect ELISA in triplicate. Error bars represent the mean ± SEM. (B,C) Neutralizing activity of the mouse anti-IFN-γ mAbs was assessed by a cell-based assay. THP-1 cells were treated with IFN-γ in the presence or absence of each mAb. The surface MHC-II expression was evaluated by flow cytometry, and the stimulation index was determined by calculating the stimulated/unstimulated ratio.