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. 2019 Aug 14;10:1905. doi: 10.3389/fimmu.2019.01905

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Copyright © 2019 Yasamut, Thongkum, Moonmuang, Sakkhachornphop, Chaiwarith, Praparattanapan, Wipasa, Chawansuntati, Supparatpinyo, Lai and Tayapiwatana.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of the epitope recognized by neutralizing anti-IFN-γ mAbs and autoAbs using biotinylated peptides and ELISA. (A) Epitope mapping of the mouse anti-IFN-γ mAb clone B27, (B) the mAb clone B133.5, and (C) the mAb clone MD-1. (D) Epitope mapping of the human anti-IFN-γ autoAbs in AOID patients, 10 serum samples from individuals with AOID, or 10 serum samples from healthy controls were pooled and tested. Epitope mapping of three of the serum samples from individuals with AOID that had strong neutralizing capacity using (E) peptide 14 and (F) peptide 15.