Figure 4.

Degranulation and cytokine release of activated NK cells. RL cells in suspension (2D) or MALC of RL cells at day 10 of culture were incubated or not with NK cells at ratio E:T 0.5:1 or 1:1 and treated or not (UT) with mAbs at 10 μl/ml. After 4 h, CD69 (A), PFN (B), CD107a (C), and IFNγ (D) expression were detected by flow cytometry by gating on CD56⁺/CD3⁻ NK cells. Results are mean ± sem of five independent experiments. ^*p < 0.05 compared to UT condition or between anti-CD20 mAbs.