Figure 9.

HER2 targeted therapy in combination with HER2-DC1 vaccine and anti-PD-1 therapy delays tumor growth and improves survival. (A) The treatment schema. (B) Balb/C mice were injected with 3 × 10⁴ TUBO cells into the mammary fat pad to create orthotopic primary tumors. TUBO bearing mice with orthotopic primary tumor were treated with class II HER2 peptide pulsed DC1 vaccine (1 × 10⁶ DC1/mouse/100 μl, twice a week for 3 weeks), anti-PD-1 antibody (two times per week) or the combination of HER2 targeted monoclonal antibodies 7.16.4 and 7.6.5 (once in a week) and class II HER2 peptide pulsed DC1 vaccine followed up with anti-PD-1 antibody twice a week. (C) Survival curve. Data shown are representative from three independent experiments and are shown as the mean number ± SEM. P-values were determined by unpaired student t-test (B) or a log-rank test (C). ***p < 0.001, **p < 0.01.