FIGURE 11.

Expression of Ccdc42 is not restricted to testis. (A) RT-PCR on cDNA generated from adult mouse testis as well as from NIH3T3 mouse fibroblasts using different primer combinations for the amplification of the full-length cDNA (exons 1-7), exons 1 + 2, and exons 6 + 7. The full-length cDNA of 1093 bp corresponding to isoform 203, as well as the 5′ exons 1 + 2 of 305 bp were exclusively amplified from testis cDNA but not from NIH3T3 cells whereas the 3′ exons 6 + 7 of 203 bp were also amplified from NIH3T3 cells. (B) Nested PCR for the detection of Ccdc42 expression. First RT-PCR on cDNA synthesized from mouse tissues to amplify exons 5-7 revealed a fragment of 535 bp in brain, testis, and epididymides. A nested PCR on the first RT-PCR products was performed with primers amplifying exons 6 + 7. In all tissues investigated, with the exception of kidney, the expected fragment of 203 bp was found. Amplification of Gapdh as control. Probes were loaded side by side on one gel but uninformative lanes were skipped from the picture as indicated by white lines.