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. 2019 Aug 14;7:151. doi: 10.3389/fcell.2019.00151

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Copyright © 2019 Tapia Contreras and Hoyer-Fender.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CCDC42 locates to manchette, perinuclear ring and connecting piece. Suspension preparations of adult mouse testis were incubated with antibodies against α-tubulin (A,E,I; green) and CCDC42 (B,F; red) and subsequently detected with the secondary antibodies anti-mouse IgG Dylight488 and anti-rabbit IgG MFP590 (A–D,I–L) or anti-mouse IgG-Dylight488 and anti-rabbit IgG-Alexa Fluor R 555 (E–H). In the control, the antibody against CCDC42 was omitted (I–L). Nuclear counterstain with DAPI (blue). Bars: 2 μm.