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. 2019 Aug 14;7:151. doi: 10.3389/fcell.2019.00151

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Copyright © 2019 Tapia Contreras and Hoyer-Fender.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Co-localization of CCDC42-Cherry and ODF1-ECFP in NIH3T3 cells. NIH3T3 cells were transfected with expression plasmids encoding either CCDC42-Cherry or ODF1-ECFP as marked on the left side. Proteins were detected either by their fluorescent tag (CCDC42-Cherry or ODF1-ECFP) or by immunostaining (anti-ODF1, anti-gamma tubulin, or anti-Pericentrin). (A–D) CCDC42-Cherry co-localizes with ODF1-ECFP when ectopically expressed. Ectopically expressed ODF1-ECFP localizes to the centrosome as verified by immunostaining using the centrosomal marker proteins γ-tubulin (E–H) or Pericentrin (I–L). Immunostaining for γ-tubulin (M–P) or Pericentrin (Q–T) demonstrates centrosomal location of CCDC42-Cherry as well. Nuclear counterstain with DAPI (blue). Bars are of 5 μm (A–D) or 2 μm (E–T).