Figure S2. IRC117539 induces AR protein degradation in prostate cancer cells.

(A) Q-PCR analyses show AR transcript levels in LNCaP prostate cancer cells. AR mRNA levels were normalized to Cyc mRNA expression. Data are presented as mean ± SEM (n = 3). (B) Western blot analysis of AR protein levels in LNCaP and 22Rv1 prostate cancer cells treated with lower doses (100 nM and 300 nM) of the IRC117539 compound (for comparison with compound’s effect at 1 μM, as shown in Fig 2A).