| Antibodies |
| Anti-mouse CD8a (53–6.7) BUV395 |
3D Bioscience |
Cat #: 563786; RRID:AB_2732919 |
| Anti-mouse CD4 (RM4–5) APC |
eBioscience |
Cat #: 17–0041-82; RRID:AB_469320 |
| Anti-mouse CD45 (30 F11) BV711 |
BD Bioscience |
Cat #: 563709; RRID:AB_2687455 |
| Anti-mouse CD45.2 (104) PerCP-Cy5.5 |
Tonbo Biosciences |
Cat #: 65–0454-U100; RRID:AB_2621894 |
| Anti-mouse CD44 (IM7) BUV737 |
BD Bioscience |
Cat #: 564392; RRID:AB_2738785 |
| Anti-mouse CD64 (X54–5/7.1) BV605 |
Biolegend |
Cat #: 139323; RRID:AB_2629778 |
| Anti-mouse Ly6C (HK1.4) APC-eF780 |
eBioscience |
Cat #: 47–5932-80; RRID:AB_2573991 |
| Anti-mouse Nkp46 (29A1.4) BV421 |
Biolegend |
Cat #: 137612; RRID:AB_2563104 |
| Anti-mouse MHC II (I:A/I:E) BV510 |
Biolegend |
Cat #: 107635; RRID:AB_2561397 |
| Anti-mouse Ly6G (1A8) BV785 |
Biolegend |
Cat #: 127645; RRID:AB_2566317 |
| Anti-mouse TLR2 (QA16A01) |
Biolegend |
Cat #: 153006; RRID:AB_2728206 |
| Anti-mouse TLR4-MD2 (MTS510) |
Biolegend |
Cat #: 117610; RRID:AB_2044020 |
| Anti-mouse IFNγ (XMG1.2) PE-Cy7 |
Tonbo Biosciences |
Cat #: 60–7311-U100; RRID:AB_2621871 |
| Anti-mouse CD62L (MEL-14) BV786 |
BD Bioscience |
Cat #: 564109; RRID:AB_2738598 |
| Anti-mouse CD14 (Sa14–2) BV421 |
Biolegend |
Cat #: 123329; RRID:AB_2721526 |
| Ghost 780 Viability Dye |
Tonbo Biosciences |
Cat#: 13–0865-T100 |
| Bacterial and Virus Strains |
| Listeria Monocytogenes Strain 10403s |
John T. Harty, University of Iowa; Hamilton et al., 2006
|
NCBITaxon: 393133 |
| Chemicals, Peptides, and Recombinant Proteins |
| LPS (from E. coli O111:B4) |
InvivoGen |
tlrl-eblps |
| Pam3CSK4 |
InvivoGen |
tlrl-pms |
| Critical Commercial Assays |
| ProcartaPlex Cytokine & Chemokine 36-plex mouse panel |
Thermo Fisher Scientific |
EPX360–26092-901; RRID:AB_2576123 |
| Mouse Custom ProcartaPlex 17-plex (CCL5, CCL7, CXCL1, CXCL5, CXCL10, GM-CSF, IFNγ, IL-1P, IL-2, IL-5, IL-6, IL-10, IL-15/IL-15R, IL-17A, IL-18, IL-28, TNFα) |
Thermo Fisher Scientific |
PPX-17-MXKA3NP |
| Mouse PCR Rodent Infectious Agent Array |
Charles River Laboratory |
N/A |
| Deposited Data |
| Raw data (16S rRNA sequencing) |
Sequence Read Archive |
BioProject accession #: SRP193509 |
| Experimental Models: Cell Lines |
| HEK-Blue hTLR4 |
InvivoGen |
Cat #: hkb-htlr4; RRID:CVCL_IM82 |
| Experimental Models: Organisms/Strains |
| Mouse: C57BL/6Ncr |
NCI Charles River |
#556 |
| Oligonucleotides |
| 16S rRNA primer set |
Claesson et al., 2010 |
BSF784/1064R |
| TaqManGene Expression Assay (FAM) -Assay ID: Mm00446095_m1; Gene Symbol: Tlr1 |
Life Technologies |
Cat #: 4453320 |
| TaqManGene Expression Assay (FAM) -Assay ID: Mm00442346_m1; Gene Symbol: Tlr2 |
Life Technologies |
Cat #: 4453320 |
| TaqManGene Expression Assay (FAM) - Assay ID: Mm01207404_m1; Gene Symbol: Tlr3 |
Life Technologies |
Cat #: 4453320 |
| TaqManGene Expression Assay (FAM) - Assay ID: Mm00445273_m1; Gene Symbol: Tlr4 |
Life Technologies |
Cat #: 4448892 |
| TaqManGene Expression Assay (FAM) - Assay ID: Mm00546288_s1; Gene Symbol: Tlr5 |
Life Technologies |
Cat #: 4453320 |
| TaqManGene Expression Assay (FAM) - Assay ID: Mm02529782_s1; Gene Symbol: Tlr6 |
Life Technologies |
Cat #: 4453320 |
| TaqManGene Expression Assay (FAM) - Assay ID: Mm00446590_m1; Gene Symbol: Tlr7 |
Life Technologies |
Cat #: 4453320 |
| TaqManGene Expression Assay (FAM) - Assay ID: Mm01157262_m1; Gene Symbol: Tlr8 |
Life Technologies |
Cat #: 4453320 |
| TaqManGene Expression Assay (FAM) - Assay ID: Mm00446193_m1; Gene Symbol: Tlr9 |
Life Technologies |
Cat #: 4453320 |
| TaqManGene Expression Assay (FAM) - Assay ID: Mm00438094_g1; Gene Symbol: Cd14 |
Life Technologies |
Cat #: 4453320 |
| TaqManGene Expression Assay (FAM) - Assay ID: Mm01227593_m1; Gene Symbol: Ly96 |
Life Technologies |
Cat #: 4448892 |
| TaqManGene Expression Assay (FAM) - Assay ID: Mm00440338_m1; Gene Symbol: Myd88 |
Life Technologies |
Cat #: 4453320 |
| TaqManGene Expression Assay (FAM) - Assay ID: Mm01260003_m1; Gene Symbol: Ticam2 |
Life Technologies |
Cat #: 4448892 |
| TaqManGene Expression Assay (FAM) - Assay ID: Mm00493836_m1; Gene Symbol: Traf6 |
Life Technologies |
Cat #: 4453320 |
| TaqManGene Expression Assay (FAM) - Assay ID: Mm01193538_m1; Gene Symbol: Irak1 |
Life Technologies |
Cat #: 4448892 |
| Software and Algorithms |
| Prism 8.0 |
Graphpad |
https://www.graphpad.com/scientific-software/prism/ |
| FlowJo |
BD Biosciences |
www.flowjo.com |
| FacsDiva |
BD Biosciences |
N/A |
| Mothur (v1.33.3) |
Schloss et al., 2009 |
https://www.mothur.org/ |
| UCHIME (version 4.2.40) |
Edgar et al., 2011 |
https://www.drive5.com/uchime/ |
| Ribosomal Database Project (version 14) |
|
https://rdp.cme.msu.edu/ |
| SourceTracker (version 0.9.8) |
Knights et al., 2011 |
https://github.com/danknights/sourcetracker/releases |
