Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 16;30(8):967–974. doi: 10.1089/hum.2018.244

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright 2019, Mary Ann Liebert, Inc., publishers

PMC Copyright notice

Figure 3. — Functional analysis of AAV2/5 IA-RPGR. (A) Fundus photograph (left) and OCT (right) of Sprague Dawley rat retina following subretinal injection. In the fundus photo, the dotted line demarcates the subretinal bleb area, and the arrow indicates the retinotomy site. In the OCT image, the arrowheads indicate the subretinal bleb (note dark fluid-filled space beneath the photoreceptor outer segments). (B) Quantitative RT-PCR analysis demonstrating expression of both IA-RPGR and RPGR ORF15 transcripts following subretinal injection in wild-type Sprague Dawley rats. (C, D) Maps of FLAG-tagged IA-RPGR and RPGR ORF15 plasmids. (E) Immunoprecipitation using FLAG-tagged RPGR ORF15G275S (mutant control), IA-RPGR, and RPGR ORF15 demonstrating that IA-RPGR-derived protein retains the ability to bind PDE6D, INPP5E, and RPGRIP1L. Anti-FLAG and anti-GAPDH antibodies were used as controls. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; OCT, optical coherence tomography; RT, reverse transcription. Color images are available online.