Figure 5.

GQ-11 increases re-epithelization and collagen deposition in wounds of db/db mice. (A) Representative sections of wounds edge of nondiabetic mice, HE stained, and the respective thickness measurement and re-epithelization calculation. (B) Representative sections of wounds edge of db/db mice, HE stained and the respective thickness measurement and re-epithelization calculation. (C) Representative sections of wounds edge of nondiabetic mice, Trichrome stained, and respective collagen deposition. (D) Representative sections of wounds edge of db/db mice, Trichrome stained, and respective collagen deposition. Mice were submitted to surgical procedure to induce scapula lesion and were treated at the 3rd day postsurgery with vehicle (CTRL—Pluronic Gel), pioglitazone (PIO—2 mM), or GQ-11 (2 mM) for 4 days (nondiabetic mice) and 7 days (db/db mice). Positive controls [CTRL (+)] are represented by wounds of db/+ mice at the 3rd day postsurgery without treatment. Histological sections were collected with frozen samples with cryostat. Magnification 2 × . Data are expressed as the mean ± SD of six mice per group. Statistical analyses were performed using ANOVA/Tukey's multiple comparison tests. *p < 0.05. HE, hematoxylin-eosin.