Figure 11.

Effects of Dock7 knockdown in mice on signaling proteins controlling myelination. Sciatic nerve tissues at 1 d old, 6 d old, and 12 d old were lysed and immunoblotted with an antibody against Oct6 (n = 3) (A), Krox20 (n = 3) (B), neuregulin-1 (NRG1) (n = 3) (C), (pSer473)Akt1 and Akt1 (n = 3) (D), (pSer16)stathmin and stathmin (n = 3) (E), or actin (n = 3) (F). The active state of Akt1 is recognized with an anti-(pSer473)Akt1 antibody. Phosphorylation of stathmin at the Ser-16 position is mediated by Rac1/Cdc42 signaling. Proteins and their phosphorylation are shown as relative values. TG, Transgenic mice; NTG, nontransgenic mice. Data were evaluated using one-way ANOVA (*p < 0.01; **p < 0.02).