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. 2011 Aug 31;31(35):12579–12592. doi: 10.1523/JNEUROSCI.2738-11.2011

Figure 3.

Figure 3.

Dock7 knockdown changes Rac1/Cdc42/JNK signaling in Schwann cells. A–D, Dock7 or luciferase siRNA-transfected Schwann cells were collected at 0–48 h following stimulation with 1 mm dibutyryl cAMP. GTP-bound Rac1 or Cdc42 was affinity precipitated with GST-CRIB, which specifically binds to GTP-bound Rac1 and Cdc42, from Schwann cell lysates, and immunoblotted with an anti-Rac1 or Cdc42 antibody. Total Rac1 and Cdc42 levels were comparable. GTP-bound Rac1 and Cdc42 are shown as relative values (n = 3). E, F, JNK1 in Schwann cell lysates was immunoprecipitated with anti-JNK1 antibody from Schwann cell lysates and immunoblotted with an anti-(pThr183/pTyr185)JNK antibody, which recognizes active JNK. Total JNK1 levels were comparable. JNK phosphorylation is shown as a relative value (n =3). G, H, Schwann cells were treated with or without 1 mm dibutyryl cAMP in the presence or absence of 10 μm JNK inhibitor I or SP600125 for 48 h. The percentages of MBP-expressing Schwann cells are shown (n = 4). Data were evaluated using one-way ANOVA (*p < 0.01; **p < 0.02).