Figure 9.

Dock7 knockdown in mice leads to enhanced sciatic nerve myelin thickness. A, Representative electron micrograph of sciatic nerve cross-sections in a 7-d-old Dock7 shRNA transgenic mouse (TG) or a nontransgenic littermate (NTG). Scale bar, 1 μm. B, To obtain statistical data, we calculated the g-ratio, that is, the numerical ratio of the diameter of the axon proper to the outer diameter of the myelinated fiber, for each axon. When a myelinated axon has a rhomboid-like form, its diameter is calculated as the average value between the longest diameter and the shortest one. Scatter plot of the g-ratio derived from electron micrographs of the sciatic nerves in transgenic mice (a total of 90 nerves in two independent mice) and nontransgenic mice (a total of 114 nerves in two independent mice) for each axonal size is shown. The average g-ratios are also shown. C, Data are presented in the form of relative distribution of g-ratios.