Table 1.

PCR primers used in the RT-PCR protocol for astrocytes and pyramidal cells

Gene accession #	First PCR primers	Size (bp)	Second PCR nested primers	Size (bp)
GFAP	Sense, 454 AGCTCCAAGATGAAACCAACCTa	631	Sense, 509 ACAGACAGGAGGCGGATGAA	263
NM_017009	Antisense, 1066 CGATCTCGATGTCCAGGGCa		Antisense, 749 GGACCGATACCACTCTTCTGTTT
S100β	Sense, 45 CATCAGTATTCAGGGAGAGAGGG	166	Sense, 58 GGGAGAGAGGGTGACAAGCA	132
NM_013191.1	Antisense, 191 GTCACACTCCCCATCCCCAT		Antisense, 170 TTCGTCCAGCGTCTCCATCA
COX-1	Sense, 27 GTTTCCCCTGCTGCTGCTC	359	Sense, 137 AGGGTGTCTGTGTCCGCTTC	185
NM_017043.1	Antisense, 366 GGCTGGGGATAAGGTTGGAC		Antisense, 301 GGCATTCACAAACTCCCAGAT
COX-2	Sense, 199 CTGAAGCCCACCCCAAACAC	247	Sense, 202 AAGCCCACCCCAAACACAGT	206
NM_017232.2	Antisense 426 TGGGAGTTGGGCAGTCATCA		Antisense, 384 CTGGTGTAGTAGGAGAGGTTGGAG
VGluT1	bSense, 361 GGCTCCTTTTTCTGGGGGTACb	259	Sense, 373 TGGGGGTACATTGTCACTCAGAb	201
NM_053859.1	Antisense, 600 CCAGCCGACTCCGTTCTAAGb		Antisense, 553 ATGGCAAGCAGGGTATGTGACb

Position 1, first base of the start codon.

^aLambolez et al., 1992.

^bGallopin et al., 2006.