Figure 3.
Inhibition of absinthin-induced calcium responses in hTAS2R46-expressing cells by 3HDC and 3HP. HEK293T Gα16gust44 cells previously transfected with hTAS2R46 cDNA or empty vector (mock) were loaded with Fluo4-AM. We recorded cytosolic calcium levels in these cells before and after bath application (arrows) of 3HDC (A; 100 μm) and 3HP (B; 100 μm) mixed with absinthin in a FLIPR. We applied absinthin at a concentration of 100 μm, which corresponds to the EC90 of hTAS2R46 (Brockhoff et al., 2007). Scale: y = 2000 counts; x = 2 min.