Figure 4.

Inhibitory properties of 3HDC and 3HP. A, B, Calcium responses of hTAS2R46-expressing cells after stimulation with various agonists are inhibited by coapplication of 100 μm 3HDC or 3HP. Agonists were applied at their EC₉₀ concentrations (abs, absinthin at 100 μm; and, andrographolide at 30 μm; den, denatonium benzoate at 300 μm; pic, picrotoxinin at 300 μm; stry, strychnine at 3 μm). C, Calcium responses of HEK293T Gα16gust44 cells after application of 100 nm somatostatin-14 in the absence or presence of 100 μm 3HDC or 3HP. D, Inhibition response curves of the effects of 3HDC (circles, solid line) or 3HP (squares, dotted line) on the absinthin-elevated intracellular calcium levels in hTAS2R46-expressing cells. E, IC₅₀ values of 3HDC and 3HP for blocking hTAS2R46 responses to various agonists. Agonists were applied at their EC₉₀ concentrations of activating hTAS2R46 (absinthin, 100 μm; andrographolide, 30 μm; denatonium benzoate, 300 μm; picrotoxinin, 300 μm; strychnine, 3 μm).