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. 2011 Oct 12;31(41):14463–14480. doi: 10.1523/JNEUROSCI.3018-11.2011

Figure 2.

Figure 2.

The DCC receptor does not coassociate with SNAP25, VAMP2, or Syntaxin 4. A, Coimmunoprecipitation experiments in brain lysates (E15 and adult). Whereas DCC and Sytx1 coimmunoprecipitate, the DCC receptor does not coassociate with SNAP25, VAMP2, or Sytx 4 in brain lysates. The Sytx antibody used for the immunoblot was an anti-Sytx1A antibody in all cases, except in the column immunoprecipitated with anti-Sytx 4 antibodies. Inputs are shown to the right. B, Coimmunoprecipitation experiments in HEK293 cells transfected with pCMVDCC and SNAP25-FLAG, VAMP2-FLAG or Sytx1A-EGFP DNAs. DCC immunoprecipitation results in coassociation with Sytx1A visualized with anti-GFP antibodies, but not with SNAP25 or VAMP2 (FLAG antibodies). The reverse immunoprecipitation with anti-FLAG antibodies did not reveal DCC coimmunoprecipitation.