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. 2011 Oct 12;31(41):14463–14480. doi: 10.1523/JNEUROSCI.3018-11.2011

Figure 7.

Figure 7.

TeNT incubation does not alter Netrin-1-induced chemoattraction. A–C, Examples of hippocampal explants cocultured with control or Netrin-1-expressing cell aggregates in the presence of TeNT. Explants were immunolabeled for βIII-tubulin. Explants cocultured with Netrin-1-secreting cells show strong chemoattraction (B). Explants cocultured with Netrin-1-secreting cells and TeNT also show strong chemoattraction (C). D, Histogram illustrating proximal/distal ratios (attraction index) in a range of conditions. Hippocampal explants cultured with Netrin-1-expressing cells in the presence or absence of TeNT show chemoattractive patterns of axonal growth. E, Western blots of protein samples from lysates of hippocampal explants cultured with TeNT, or left untreated (Ctrl), probed with α-VAMP2 antibody. Incubation with TeNT cleaves VAMP2. Significant differences are labeled by asterisks (**p ≤ 0.001). Scale bar: A, D, 150 μm. Error bars indicate SEM.