TABLE 3.

Differential expression of genes encoding methanol methyltransferase enzymes in M. acetivorans cells^a

Locus	Annotation	Gene	Fold upregulationb	P	FDR
MA4379	Co-methyl-5-hydroxybenzimi- dazolylcobamide:2-mercapto-ethanesulphonic acid methyltransferase, isozyme 1	mtaA1	–1.68	0.01	0.02
MA0455	Methanol:5-hydroxybenzimidazolyl-cobamide methyltransferase, isozyme 1	mtaB1	–6.84	0.02	0.04
MA0456	Corrinoid-containing methyl-accepting protein, isozyme 1	mtaC1	–7.95	0.01	0.03
MA4392	Methanol:5-hydroxybenzimidazolylcobamide methyltransferase, isozyme 2	mtaB2	68.55	5.70 × 10–11	2.56 × 10–7
MA4391	Corrinoid-containing methyl-accepting protein, isozyme 2	mtaC2	48.28	3.27 × 10–10	5.54 × 10–7
MA1615	Co-methyl-5-hydroxybenzimidazolylcobamide:2- mercapto-ethanesulphonic acid methyltransferase, isozyme 2	mtaA2	5.39	1.77 × 10–7	8.04 × 10–6
MA1616	Methanol:5-hydroxybenzimidazolylcobamide methyltransferase, isozyme 3	mtaB3	9.66	5.24 × 10–8	4.89 × 10–6
MA1617	Corrinoid-containing methyl-accepting protein, isozyme 3	mtaC3	8.49	2.52 × 10–7	1.00 × 10–5

^aCells were grown with methanol provided as an electron donor and AQDS provided as an electron acceptor in the presence of BES or cells grown via methanogenesis with methanol as the substrate. Negative values indicate that genes were more significantly expressed in methanogenic cells. Genes were only considered differentially expressed if the P value and FDR were ≤0.05.

^bThat is, in AQDS/BES versus methanogenesis.