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. 2019 Aug 20;10(4):e01557-19. doi: 10.1128/mBio.01557-19

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Copyright © 2019 Heinrich et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Late-stationary-phase cell filaments remain with mainly one or two chromosomes. (A) Flow cytometry profiles of cells from exponential-phase growth and early-stationary-phase growth in PYEX and small cells and filamentous cells after purification stained with SYTOX Green to measure the DNA content. Indicated are the fluorescent intensities corresponding to one chromosome (1N) and two chromosomes (2N). (B) Overlay of phase-contrast and fluorescence microscopy images of strain MT16 carrying a (tetO)_n array near the origin of replication fluorescently labeled with TetR-YFP after 10 days in PYEX. Arrowheads indicate fluorescently labeled origins. Quantification data at left show the number of foci in 206 filamentous cells.