FIG 3.

Phylogenetic affiliation of “Ca. Syntrophoarchaeum” and “Ca. Methanoliparia” based on the 16S rRNA gene. (A) Phylogenetic tree of the 16S rRNA gene of the phylum Euryarchaeota, with a focus on Methanomicrobia. Sequences of the “Ca. Methanoliparia” clade are shown in red, and sequences of the “Ca. Syntrophoarchaeum” clade are shown in purple. (B) Extended display of the clades of interest, with 16S rRNA full-length gene sequences of the “Ca. Methanoliparia” and “Ca. Syntrophoarchaeum” bins in bold. (C) Phylogeny of the “Ca. Methanoliparia” clade based on the 16S rRNA gene, including full-length sequences (in bold) and representative short sequences from studies that detected “Ca. Methanoliparia” in SILVA database v132. The tags refer to accession numbers, clone identifiers (when applicable), lengths, and isolation origins. On the right, the classifications of sequences according to their environmental origins are shown. For all panels, scale bars represent the number of nucleotide substitutions per site. For panels A and B, bootstrap values over 50% and 90% are indicated with gray and black circles, respectively.