FIG 4.

FKBP protein destabilization domain validates that HCMV US28 is required for viral reactivation. CD34⁺ HPCs were infected with HCMV TB40E-GFP-WT or -US28-ddFKBP and cultured to establish latency as described for Fig. 3. Following the establishment of latency, equal numbers of cells were either cocultured with NHDFs with or without 1 μM Shield-1 (reactivation conditions) or lysed and plated onto NHDFs (prereactivation). At 14 days postplating, the number of GFP-positive wells was determined by fluorescence microscopy, and the frequency of infectious centers was determined by ELDA software.