Figure 5:

Changes in the levels of stress-responsive metabolites in flag leaves at the early grain-filling stage after rewatering (RW). Venn diagrams show the number of common and cultivar-specific metabolites that showed a significant (Mann-Whitney-Wilcoxon test, P < 0.05) increase (A, C, E) or decrease (B, D, F) in levels 12 hours (A, B), 36 hours (C, D), and 60 hours (E, F) after RW relative to severe stress conditions. Numbers in parentheses indicate the total number of metabolites with increased/decreased abundance in each cultivar. The corresponding metabolites are shown in the heat map (G). The values, expressed as log₂-fold change between plants after RW and plants under severe stress, are indicated by the color code and hierarchically clustered using Euclidean distance and mean linkage. Asterisks indicate the level of significance (* P < 0.05; ** P < 0.01; *** P < 0.001).