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. 2012 Mar 14;32(11):3917–3930. doi: 10.1523/JNEUROSCI.6165-11.2012

Figure 4.

Figure 4.

L1 interacts with MMP14 and GAPDH interacts with ANT. A, B, A detergent-solubilized brain homogenate was subjected to immunoprecipitation using ANT1- or ANT2-specific antibodies (A), polyclonal L1 (B), and nonimmune rabbit (rbIg) antibodies (A, B). Brain homogenate (input) and immunoprecipitates (IP) were subjected to Western blot (WB) analysis using monoclonal GAPDH (A) or MMP14 antibodies (B). C, D, Binding of GAPDH to ANT was determined by ELISA with substrate-coated GAPDH and different concentrations of soluble ANT (C) or by label-free binding assay using substrate-coated ANT and different concentrations of soluble L1-Fc (D). E, Binding of L1 to MMP14 was determined by label-free binding assay using substrate-coated MMP14 and different concentrations of soluble L1-Fc and of Fc control. Mean values ± SD from triplicates of a representative experiment are shown (C–E). The experiments were repeated twice with identical results.