Table 1.
Mass spectrometry of the 32 kDa protein band isolated by immunoprecipitation with immobilized L1 antibody revealed seven and five masses that match with mouse ANT1 and ANT2, respectively
| m/z submitted | MH+ matched | ΔDa | Start | End | Missed cleavages | Peptide sequence | |
|---|---|---|---|---|---|---|---|
| ANT1 | 902.480 | 902.484 | −0.004 | 273 | 280 | 0 | GAWSNVLR |
| 1004.580 | 1004.552 | 0.028 | 97 | 105 | 0 | QIFLGGVDR | |
| 1169.580 | 1169.574 | 0.006 | 64 | 72 | 0 | EQGFLSFWRa | |
| 1205.600 | 1205.584 | 0.016 | 189 | 199 | 0 | AAYFGVYDTAK | |
| 1219.640 | 1219.668 | −0.028 | 11 | 23 | 0 | DFLAGGIAAAVSK | |
| 1446.640 | 1446.742 | −0.102 | 81 | 92 | 0 | YFPTQALNFAFK | |
| 1755.820 | 1755.935 | −0.115 | 281 | 296 | 1 | GMGGAFVLVLYDEIKK | |
| ANT2 | 902.480 | 902.484 | −0.004 | 273 | 280 | 0 | GAWSNVLR |
| 1121.580 | 1121.574 | 0.006 | 64 | 72 | 0 | EQGVLSFWRa | |
| 1219.640 | 1219.668 | −0.028 | 11 | 23 | 0 | DFLAGGIAAAVSK | |
| 1446.640 | 1446.742 | −0.102 | 81 | 92 | 0 | YFPTQALNFAFK | |
| 1755.820 | 1755.935 | −0.115 | 281 | 296 | 1 | GMGGAFVLVLYDEIKK |
aSequence was confirmed by post-source decay fragment ion mass analysis.