Expression of c-Maf and its target genes in the dorsal horn of MafA−/− mice. A, B, Immunostaining of c-Maf was performed on sections of spinal cord in MafA+/− and MafA−/− mice at P0. Insets show the anti-c-Maf immunofluorescence signal in the DRG of control and MafA deletion mice. Note comparable expression of c-Maf in both the dorsal horn and the DRG in MafA−/− versus control mice. C–H, In situ hybridization was performed with Gabra5 (C, D), CCK (E, F), and Rora (G, H) probes on sections of cervical/thoracic spinal cord in MafA+/− and MafA−/− mice at P0. No apparent difference was observed in MafA mutant and control mice. Scale bars: (in B, H) A–H, 50 μm; (in B, inset), A, B, insets, 50 μm.